Waddlia chondrophila induces systemic infection, organ pathology, and elicits Th1-associated humoral immunity in a murine model of genital infection.

Waddlia chondrophila is a known bovine abortigenic Chlamydia-related bacterium that has been associated with adverse pregnancy outcomes in human. However, there is a lack of knowledge regarding how W. chondrophila infection spreads, its ability to elicit an immune response and induce pathology. A murine model of genital infection was developed to investigate the pathogenicity and immune response associated with a W. chondrophila infection. Genital inoculation of the bacterial agent resulted in a dose-dependent infection that spread to lumbar lymph nodes and successively to spleen and liver. Bacterial-induced pathology peaked on day 14, characterized by leukocyte infiltration (uterine horn, liver, and spleen), necrosis (liver) and extramedullary hematopoiesis (spleen). Immunohistochemistry demonstrated the presence of a large number of W. chondrophila in the spleen on day 14. Robust IgG titers were detected by day 14 and remained high until day 52. IgG isotypes consisted of high IgG2a, moderate IgG3 and no detectable IgG1, indicating a Th1-associated immune response. This study provides the first evidence that W. chondrophila genital infection is capable of inducing a systemic infection that spreads to major organs, induces uterus, spleen, and liver pathology and elicits a Th1-skewed humoral response. This new animal model will help our understanding of the mechanisms related to intracellular bacteria-induced miscarriages, the most frequent complication of pregnancy that affects one in four women

Study of supports for the final doublets of ATF2

We investigated supports for the final doublets of ATF2 with vertical relative motion to the floor of final doublets below 10nm. Our calculations of relative motion were done by using data of ATF ground motion. We studied the vibratory behaviour of a steel lightweight honeycomb table as a base for fixing magnets. First, the table was fixed to the floor by four steel feet at its corners. Its first vertical resonance was at 41Hz, which induces a non negligible relative motion (5.7nm) compared to ATF2 tolerances. Modal shape measurements show that the six first resonances of the table (below 150Hz) are rigid body modes in the six degrees of freedom. The conclusion of these measurements is that the table is very rigid and well adapted for ATF2 project but the rigidity of the four steel feet is not sufficient compared to the rigidity of the table. Consequently, the table was fixed to the floor on one entire face to break these six rigid body modes by three large steel plates. The first vertical resonance was then at higher frequencies (92Hz), which show that good boundary conditions were chosen for the table. The relative motion was then low (3.5nm above 0.1Hz) compared to ATF2 tolerances. To finish, we studied the vibratory behaviour of one ATF2 FD sextupole and one ATF2 FD quadrupole with their intermediary supports made at LAPP and used to fix these magnets to the honeycomb table. The measurements showed that the final doublets with their intermediary supports were well designed because the first resonance of sextupoles and quadrupoles was at high frequency (above 100 Hz and at 76Hz respectively), which induced a small relative motion of final doublets to the floor compared to ATF2 tolerances

Anomalous electric conductions in KSbO3-type metallic rhenium oxides

Single crystals of KSbO3-type rhenium oxides, La4Re6O$19, Pb6Re6O19, Sr2Re3O9 and Bi3Re3O11, were synthesized by a hydrothermal method. Their crystal structures can be regarded as a network of three-dimensional orthogonal-dimer lattice of edge-shared ReO6 octahedra. All of them exhibit small magnitude of Pauli paramagnetism, indicating metallic electronic states without strong electron correlations. The resistivity of these rhenates, except Bi3Re3O11, have a temperature dependence of$rho(T)=\rho_{0}+AT^{n}$$(n \approx 1.6)$ in a wide temperature range between 5 K and 300 K, which is extraordinary for three-dimensional metals without strong electron correlations. The resistivity of Bi3Re3O11 shows an anomaly around at 50 K, where the magnetic susceptibility also detects a deviation from ordinary Pauli paramagnetism.Comment: 13 pages, 7 figures. J. Phys. Soc. Japan, in pres

What is the true clinical relevance of Simkania negevensis and other emerging Chlamydiales members?

<i>Waddlia</i>   <i>chondrophila</i> and <i>Simkania negevensis</i> are emerging <i>Chlamydia-</i> related bacteria. Similar to the pathogenic organisms <i>Chlamydia pneumoniae</i> and <i>Chlamydia trachomatis,</i> these emerging bacteria are implicated in human genital infections and respiratory diseases. We used a screening strategy based on a newly developed <i>S. negevensis</i> -specific quantitative real-time PCR (qPCR) and a pan- <i>Chlamydiales</i> qPCR. We could not detect <i>S. negevensis</i> in 458 respiratory, genitourinary, cardiac and hepatic samples tested. One urethral swab was positive for <i>W. chondrophila.</i> We observed a low prevalence of <i>Chlamydiales</i> in respiratory samples (1/200, 0.5%), which suggests that <i>C. pneumoniae</i> is an uncommon respiratory pathogen. Furthermore, we screened 414 human serum samples from Switzerland, England and Israel and observed a low prevalence (<1%) of exposure to <i>S. negevensis.</i> Conversely, humans were commonly exposed to <i>W. chondrophila,</i> with seroprevalences ranging from 8.6% to 32.5%. <i>S. negevensis</i> is not a clinically relevant pathogen, but further research investigating the role of <i>W. chondrophila</i> is needed

The distribution of maser stars in the inner Milky Way: the effect of a weak, rotating bar

We derive the distribution of maser stars in the inner Milky Way (MW) based on an analysis of lV-diagrams (lVd) for two samples of maser stars: 771 OH/IR stars and 363 SiO-maser stars. They are all close to the plane of the MW and have long. from -45 to +45deg. The two lVds are compared and found to be very similar. They also compare well with the lVd of interstellar CO, but there are significant differences in detail between the stellar lVds and that of the ISM. Based on the qualitative discussion we divide the lVds into seven areas. In each area we compare the number of stars observed with those predicted by an assumed set of orbits in a galactic potential. This potential is axially symmetric but a weak rotating bar has been added. We conclude that the maser stars move on almost circular orbits outside of about 3.5 kpc, but that the orbits become more and more elongated when one goes deep inside our MW. We find a strong effect of the Corotation (CR) resonance (res) at 3.3 kpc, we see a small but noticeable effect of the Outer Lindblad res at 5 kpc and no effect of the Inner Lindblad res at r=0.8 kpc. We find a set of 6 groups of orbits that together predict counts in agreement with the counts of stars observed. We then calculate the trajectory of each orbit and so find the distribution of the maser stars in the plane of the MWG. This distribution has two new (but not unexpected) features. The first is a bar-like distribution within 2 kpc from the GC outlined. These orbits explain the high-vel stars near l=0deg in the forbidden and the permitted quadrants. The second feature are two "croissant"-like voids in the distribution close to the CR radius (3.3 kpc), which are the consequence of the presence of the CR res. We find excellent agreement with an earlier reconstruction by Sevenster (1999)

Spatial Diffusion of Stars in the Inner Galactic Bulge

Star formation in the inner few hundred pc of the Galactic bulge occurs in a flattened molecular layer called the central molecular zone (CMZ). Serabyn & Morris (1996) suggest that the star formation in the CMZ has been sustained for the lifetime of the Galaxy, and that the resulting agglomeration of stars formed in the CMZ has resulted in the prominent r^-2 stellar density cusp at the Galactic center having about the same physical extent as the CMZ. This "central cusp" is somewhat less flat than the CMZ; thus the population of stars formed in the CMZ appears to have diffused out to larger latitudes. We hypothesize that such vertical diffusion is driven by the scattering of stars off the giant molecular clouds (GMC) in the CMZ, and perform numerical simulations of the scattering between stars and GMCs in the presence of the non-axisymmetric background potential. The simulation results show that the time scale for an initially flattened stellar population to achieve an aspect ratio of the observed OH/IR stars in the inner bulge, 1 to 2 Gyr, agrees well with the estimated age of those OH/IR stars.Comment: To appear in ApJ, Jun 2001 issu

GASKAP -- The Galactic ASKAP Survey

A survey of the Milky Way disk and the Magellanic System at the wavelengths of the 21-cm atomic hydrogen (HI) line and three 18-cm lines of the OH molecule will be carried out with the Australian Square Kilometre Array Pathfinder telescope. The survey will study the distribution of HI emission and absorption with unprecedented angular and velocity resolution, as well as molecular line thermal emission, absorption, and maser lines. The area to be covered includes the Galactic plane (|b|< 10deg) at all declinations south of delta = +40deg, spanning longitudes 167deg through 360deg to 79deg at b=0deg, plus the entire area of the Magellanic Stream and Clouds, a total of 13,020 square degrees. The brightness temperature sensitivity will be very good, typically sigma_T ~ 1 K at resolution 30arcsec and 1 km/s. The survey has a wide spectrum of scientific goals, from studies of galaxy evolution to star formation, with particular contributions to understanding stellar wind kinematics, the thermal phases of the interstellar medium, the interaction between gas in the disk and halo, and the dynamical and thermal states of gas at various positions along the Magellanic Stream.Comment: 45 pages, 8 figures, Pub. Astron. Soc. Australia (in press

What Cognitive and Affective States Should Technology Monitor to Support Learning?

This paper discusses self-efficacy, curiosity, and reflectivity as cognitive and affective states that are critical to learning but are overlooked in the context of affect-aware technology for learning. This discussion sits within the opportunities offered by the weDRAW project aiming at an embodied approach to the design of technology to support exploration and learning of mathematical concepts. We first review existing literature to clarify how the three states facilitate learning and how, if not supported, they may instead hinder learning. We then review the literature to understand how bodily expressions communicate these states and how technology could be used to monitor them. We conclude by presenting initial movement cues currently explored in the context of weDRAW

The K^{+}--->\pi^{+}\nu\bar{nu} Rare Decay in Two Higgs Doublet Model

The rare k+--->pi+ \nu \bar{\nu} decay is investigated in the context of type II two-Higgs-doublet model (2HDM). By using the existing experimental data of the branching ratio, restrictions on the free parameters of the model m_H, and tan\beta are obtained: tan\beta belong to the range 0.7- o.8, and m_H belong to the range 500- 700 GeV.Comment: 12 pages, latex file, 3 figures, postscript file

Inhibition of Protein Kinase C-Driven Nuclear Factor-κB Activation: Synthesis, Structure−Activity Relationship, and Pharmacological Profiling of Pathway Specific Benzimidazole Probe Molecules

A unique series of biologically active chemical probes that selectively inhibit NF-κB activation induced by protein kinase C (PKC) pathway activators have been identified through a cell-based phenotypic reporter gene assay. These 2-aminobenzimidazoles represent initial chemical tools to be used in gaining further understanding on the cellular mechanisms driven by B and T cell antigen receptors. Starting from the founding member of this chemical series 1a (notated in PubChem as CID-2858522), we report the chemical synthesis, SAR studies, and pharmacological profiling of this pathway-selective inhibitor of NF-κB activation